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PCR Troubleshooting and Optimization: The Essential Guide

CONTENTS

1. Magic in solution: an indtroduction and brief history of pCR 1
Carl T. Wittwer and Jared S. Farrar

2. ...

  • CodeCallNoLokasiKetersediaan
    01021110500087Tersedia
  • Perpustakaan
    Fakultas Farmasi
    Judul Seri
    -
    No. Panggil
    610.28 Ken P
    Penerbit Caister Academic Press : UK.,
    Deskripsi Fisik
    vii, 235 hal. : ilus. ; 24 cm Index. Hal. 233-235
    Bahasa
    Indonesia
    ISBN/ISSN
    978-1-904455-72-1
    Klasifikasi
    610.28 Ken P
    Tipe Isi
    -
    Tipe Media
    -
    Tipe Pembawa
    -
    Edisi
    NULL
    Subyek
    Title
    PCR
    Info Detil Spesifik
    -
    Pernyataan Tanggungjawab
  • CONTENTS

    1. Magic in solution: an indtroduction and brief history of pCR 1
    Carl T. Wittwer and Jared S. Farrar

    2. qPCR inhibition and amplication of difficult templates 23
    Jack M. Gallup

    3. Significance of controls and standard curves in PCR 67
    Ian Kavanagh, Gerwyn Jones and Saima N. Nayab

    4. Obtaining maximum PCR sensitivity and specificity 79
    Cameron N. Gundry and Matthew D. Poulson

    5. RT-PCR optimization strategies 97
    Martina Reiter and Michael W. Pfaffl

    6. Real-time PCR instrumetation: an instrument selection guide 119
    Sandrina Javorschi-Miller and ivan Delgado Orlic

    7. Quantitative PCR data analysis-unlocking the secret to successful results 139

    8. The MiQE guidelines uncloaked 151
    Greg Shipley

    9. PCR applications for epigenetics research 167
    Gavin Meredith, Miroslav Dudas, Mark Landers, Vasiliki Anest, Jonathan Wang, Caifu Chen, Peter Jozsi and Christopher Adams

    10. High-resolution melting analysis 201
    Jhon F, Mackay and Carl T. Wittwer

    11. Microfluidic emulsion PCR 217
    N. Reginald Beer and jHon L. leamon

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