Perpustakaan Universitas Padjajaran
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D102- Characterization and Anticancer Mechanism of Indonesian West Java Coffee Bean Extracts Against Human Breast Cancer MCF-& and MDA-MB-231 Cells (Mohamed Almabrouk Aborziza; Prof. Muchtaridi, Ph.D; Dr. Ade Zuhrotun, M.Si; Riezki Amalia, Ph.D)

Coffee bean extracts have demonstrated significant biological effects on humancancer cells. Epidemiological and experimental studies suggest that ...

  • CodeCallNoLokasiKetersediaan
    FFUP20260166D102Tersedia
  • Perpustakaan
    Fakultas Farmasi
    Judul Seri
    -
    No. Panggil
    D102
    Penerbit Fakultas Farmasi Universitas Padjadjaran : Jatinangor.,
    Deskripsi Fisik
    -
    Bahasa
    English
    ISBN/ISSN
    -
    Klasifikasi
    D102
    Tipe Isi
    -
    Tipe Media
    -
    Tipe Pembawa
    -
    Edisi
    -
    Subyek
    -
    Info Detil Spesifik
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    Pernyataan Tanggungjawab
  • Coffee bean extracts have demonstrated significant biological effects on humancancer cells. Epidemiological and experimental studies suggest that regular coffee consumption may contribute to the prevention and potential treatment of cancer by inhibiting its initiation and progression. Bioactive compounds found in coffee, particularly chlorogenic acid and caffeine, exhibit notable anti-breast cancer activities across various cellular models. The etiology of breast cancer is complex and multifactorial, involving interactions among genetic, behavioral, and environmental factors, as well as the influence of hormone-related subtypes. This study aims to characterize West Java coffee bean extracts, to determine their phytochemical composition, and to quantify caffeine and chlorogenic acid. Additionally, the research evaluated the anti-breast cancer mechanisms of coffee bean extracts on MDA-MB-231 and MCF-7 cell lines. A comprehensive extraction method and phytochemical profiling of green and medium-roasted Arabica and Robusta coffee beans from West Java, Indonesia, was conducted using HPLC to quantify caffeine and chlorogenic acid. Cytotoxicity and IC50 values were determined via the WST-8 assay. MYC and AXIN2 gene expression were examined by qPCR to assess their roles in cancer pathways. Cytotoxicity assays on normal HEK 293 cells were performed with extracts showing the highest inhibitory effects. Flow cytometry characterized apoptosis and cell cycles in MDA-MB-231 and MCF-7 breast cancer cells after treatment. Molecular mechanisms were explored with docking, 200-ns molecular dynamics, and MM-PBSA binding free energy calculations targeting Tankyrase-1 and Estrogen Receptor Alpha. The results showed that the yield of roasted coffee extract was slightly higher than that of green coffee extract, and the decoction method (range 18.54%-21.62%) yielded more extract than the maceration method (range 16.82%- 19.18%-). Roasting and extraction methods affected phytochemicals, with higher temperatures reducing chlorogenic acid levels and increasing caffeine levels. The CA level range in green coffee extracts is (174.61 - 203.49 mg/L), while the CA level range in roasted coffee extracts is (39.28 – 60.21 mg/L). The CF level ranged in green coffee extracts is (49.30 – 111.74 mg/L) and in roasted coffee extracts is (52.83 – 147.33 mg/L). Coffee extracts showed cytotoxicity against MDA-MB-231 (IC₅₀ 258.4–637.0 μg/mL) and MCF-7 (IC₅₀ 190.7–739.1 μg/mL) cells, and minimal toxicity to HEK293 cells. Green Arabica and Robusta extracts, via maceration, notably downregulated MYC and slightly reduced AXIN2. Flow cytometry indicated increased late apoptosis and cell-cycle arrest. Docking analysis found that chlorogenic acid binds more strongly to Tankyrase-1 than Erα (In Tankyrase-1 the native ligand demonstrated a strong binding affinity of −12.27 kcal/mol with a nanomolar inhibition constant, whereas chlorogenic acid showed a lower binding energy of −8.08 kcal/mol with a micromolar Ki value while in Erα the native ligand 4-hydroxytamoxifen exhibited a binding affinity of −11.41 kcal/mol, whereas chlorogenic acid showed a weaker affinity of −6.07 kcal/mol with a micromolar Ki). MD and MM-PBSA confirmed stable, energetically favorable binding to Tankyrase-1 complex. The conclusion is coffee bean extracts, especially chlorogenic acid–rich green coffee, show selective antiproliferative effects in breast cancer cells and may act through Wnt signaling rather than estrogen receptors. More in vivo and mechanistic studies are needed to confirm their potential as complementary or preventive anticancer agents.
    Keywords: breast cancer, coffee extract, chlorogenic acid, caffeine, MYC, AXIN2,MCF-7, and MDA-MB-231

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